Figure 5

Galectin-3-induced ROS-production in neutrophils from cord blood obtained after Caesarean section or vaginal delivery. Isolated cord blood neutrophils were kept on ice or incubated at 37°C in the absence or presence of LPS before addition of galectin-3 (at time = 0 min). (A) Representative traces of ecROS and icROS production (y-axis, Mcpm; observe the different axes in upper, middle, and lower panels), measured over time (x-axis, minutes) by CL, are shown for neutrophils from Caesarean section (black lines) and vaginal delivery (dotted lines). (B) Comparisons of galectin-3-induced peak ecROS (upper panel) and icROS (lower panel) responses between cord blood neutrophils from Caesarean section (light grey; n = 10) and vaginal delivery (white; n = 10) after the different incubations are shown. Statistical analysis was performed using the Mann–Whitney test (*p < 0.05; **p < 0.005; ***p < 0.001).