Galectin-3-induced ROS production. Isolated neutrophils were kept on ice or incubated at 37°C in the absence or presence of LPS before addition of galectin-3 (at time = 0 min). (A) Representative traces of ecROS and icROS production (y-axis, Mcpm; observe the different axes in upper, middle, and lower panels) measured over time (x-axis, minutes) by CL in neutrophils isolated from an adult control (dashed line) and cord blood drawn after a Caesarean section (solid line) are shown. (B) Comparisons of galectin-3-induced peak ROS responses between neutrophils isolated from adults (dark grey; n = 10) and cord blood after Caesarean section (light grey; n = 10) following the different incubations are shown for ecROS (upper panel) and icROS (lower panel). Statistical analysis was performed using the Mann–Whitney test (*p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001).