Sample (parameter) | Description | Purpose | 1sttrimester † | 2ndtrimester | 3rdtrimester | 1-2 days postnatal | 3-5 days postnatal | 3 m | 6 m | 9, 15, 21, 30 m | 12, 18, 24, 36 m | Proband |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Informed consent | - | Informed consent | * | * | ||||||||
Demographics (including ancestry, socioeconomic status) | - | Maternal | *ψ | |||||||||
Paternal | *ψ | |||||||||||
Infant | *ψ | |||||||||||
Proband | * | |||||||||||
Medical/obstetric history | - | Maternal | * | * | * | |||||||
Paternal | *ψ | |||||||||||
Proband | * | |||||||||||
OGTT results | * | |||||||||||
Pregnancy complications | *ψ | |||||||||||
Labour and delivery | *ψ | |||||||||||
Congenital abnormalities | *ψ | |||||||||||
Anthropometry | - | Height, Weight, BMI | * | * | * | * | ||||||
Weight z, length z, BMI z | * | * | * | * | * | |||||||
Waist circumference | * | * | * | * | * | |||||||
Lifestyle questionnaire | * | * | * | * | * | * | * | |||||
Blood | A: Serum (red) | AutoAbs: Melbourne | * | *Δ | * | * | * | * | ||||
AutoAbs: Adelaide | * | *Δ | * | * | * | * | ||||||
Resistance: adiponectin, hsCRP, C-peptide, insulin, glucose | * | * | * | *Δ | * | * | * | * | ||||
Metabolome/lipidome | * | * | * | *Δ | * | * | * | * | ||||
B: Serum (red) | Vitamin D (25 OHD) / tTGAb / C-peptide / glucose | 25 OHD tTGAb | 25 OHD tTGAb | 25 OHD tTGAb | 25 OHD tTGAb C-peptideΔ | 25 OHD tTGAb | tTGAb | 25 OHD tTGAb | tTGAb glucose (12, 24, 36 m) | |||
C: LH (green) | Cytokines/chemokines (innate immunity) | * | * | * | * | |||||||
D: K-EDTA (purple) | Buffy coat (epigenome) | * | * | * | *Δ | * | * | * | * | |||
RBC | * | * | * | *Δ | * | * | * | * | ||||
Virology | * | * | * | *ΔΩ | * | * | * | * | ||||
DHA, EPA, resolvins, protectins, prostaglandin E2 | * | * | * | *Δ | * | * | ||||||
Advanced glycation end products | * | * | * | *Δ | * | * | ||||||
Haemoglobin A1C (HbA1c) | *†† | *†† | *†† | *Δ | *ϕ | *ϕ | ||||||
E: K-EDTA (purple) | HLA typing | *Δ | * | |||||||||
F: CPT sodium citrate (blue/black) | PBMCs (adaptive immunity) | *Δ | * | * | * | |||||||
Urine | Maternal: urine pot Infant: collection bag | Metabolome/lipidome | * | * | * | * | * | * | * | * | ||
Advanced glycation end products | * | * | * | * | * | * | ||||||
Stool | Maternal: EasySampler Infant: stool collection kit | Microbiome | * | * | * | * | * | * | * | * | ||
Virology | * | * | * | * | * | * | * | * | ||||
Metabolome/lipidome | * | * | * | * | * | * | * | * | ||||
Saliva | Oragene kit | SNP typing | * | * | ||||||||
Tongue dorsum | ESwab swab | Microbiome | * | * | * | * | * | * | * | * | ||
Buccal mucosa | ESwab swab | Microbiome | * | * | * | * | * | * | * | * | ||
Throat | UTM | Virology | * | * | * | * | * | * | * | * | ||
Nasal | ESwab swab | Microbiome | * | * | * | * | * | * | * | * | ||
Skin (inner elbows) | ESwab swab | Microbiome | * | * m&i | * m&i | * m&i | * m&i | * | * | |||
Vagina | ESwab swab | Microbiome | * | |||||||||
Colostrum (day 0–3) | Colostrum collection kit | Microbiome | * | |||||||||
Metabolome/lipidome | * | |||||||||||
Breast milk | Milk collection kit | Microbiome | * | *ζ | *ζ | *ζ | *ζ | |||||
Metabolome/lipidome | * | *ζ | *ζ | *V3 ζ | ||||||||
Diet | Maternal diet evaluation tools | DQES version 2 | * | *ζ | ||||||||
Infant diet evaluation tools | Infant diary (birth-6 m) | * | * | |||||||||
Infant diary (6-12 m) | * V3 | * V4 | ||||||||||
24 hour recall (12 – 24 months) | * V5, V7 | * V6 | ||||||||||
Australian Toddler Eating Survey (>24 months) | * V9 | * V8, V10 |