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Figure 1 | BMC Pediatrics

Figure 1

From: Circulating hematopoietic stem cell count is a valuable predictor of prematurity complications in preterm newborns

Figure 1

A representative study of gating strategy for analyzing/sorting of non-HSCs/VSELs, HSCs, and CSPCs circulating in newborn PB by FACS. Each colored gate illustrates a population of cells which expresses selected surface proteins. Panel A: Cytofluorimetric analysis of non-HSCs and HSC: CD184+lin- cells were gated from a total fraction of immunofluorescence-stained nucleated cells from the PB of preterm and full term infants, then those cells from region R1 were further analyzed for CD45 expression; Panel B: Cytofluorimetric analysis of CSPCs (CD133+CD34+ and CD133-CD34+): CD34+ cells were gated from a total fraction of immunofluorescence-stained nucleated cells from the PB of preterm and full term infants, then those cells from region R2 were further analyzed for CD133 expression.

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