Figure 1

A representative study of gating strategy for analyzing/sorting of non-HSCs/VSELs, HSCs, and CSPCs circulating in newborn PB by FACS. Each colored gate illustrates a population of cells which expresses selected surface proteins. Panel A: Cytofluorimetric analysis of non-HSCs and HSC: CD184⁺lin^- cells were gated from a total fraction of immunofluorescence-stained nucleated cells from the PB of preterm and full term infants, then those cells from region R1 were further analyzed for CD45 expression; Panel B: Cytofluorimetric analysis of CSPCs (CD133⁺CD34⁺ and CD133^-CD34⁺): CD34⁺ cells were gated from a total fraction of immunofluorescence-stained nucleated cells from the PB of preterm and full term infants, then those cells from region R2 were further analyzed for CD133 expression.