Table 2 Gender, age, diagnosis, species presence and microscopic evidence of biofilm and intracellular bacteria using Fluorescence In Situ hybridisation (FISH) in middle ear mucosal biopsies and effusions assayed using culture, PCR and FISH methods for pathogen detection.
From: Multi-species bacterial biofilm and intracellular infection in otitis media
| Â | Â | Â | Â | Â | PCR | FISH | Â | Â | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Child No. | Sex (Age, yr) | Ear | Diagnosis | Current Abx | Culture | Spn | EUB | Spn | Hi | Mcat | Sau | Biofilm | Intracellular bacteria |
Children with recurrent acute otitis media | |||||||||||||
4 | F (1.4) | R | RAOM | N | - | - | + | na | + | + | na | + | + |
5 | M (1.8) | L | RAOM | N | na | na | + | - | + | na | na | - | + |
6 | F (3.2) | L | RAOM | N | na | na | + | na | + | - | na | + | + |
7 | M (6.1) | L | RAOM | N | + mixed | + | + | + | na | - | na | - | + |
8 | F (9.3) | L | RAOM, Cholesteatoma | N | na | na | + | - | na | + | na | - | - |
Children with recurrent acute otitis media and otitis media with effusion | |||||||||||||
9 | M (1.4) | R | RAOM, OME | Y | - | - | + | na | - | + | na | + | + |
10 | M (1.8) | L | RAOM, OME | N | + Spn, Mcat | + | + | + | na | + | na | + | + |
11 | F (1.8) | L | RAOM, OME | Y | - | + | + | - | + | na | na | - | + |
12 | F (3.5) | L | RAOM, OME | N | - | - | - | na | - | - | na | - | - |
13 | M (7.5) | L | RAOM, OME | N | - | - | + | na | + | + | na | + | + |
14 | F (8.9) | L | RAOM, OME | N | na | na | + | + | - | na | na | + | + |
Children with otitis media with effusion | |||||||||||||
15 | M (1.5) | L | OME | N | na | na | + | - | - | na | na | + | - |
16 | F (5.4) | L | OME | N | + CNS | - | + | na | - | + | na | + | + |
17 | F (6.0) | L | OME | N | + Sau | - | + | na | na | - | + | + | + |
18 | F (6.3) | R | OME | N | na | na | - | - | na | - | na | - | - |
19 | F (8.0) | R | OME | N | na | na | + | - | - | na | na | + | - |
20 | M (9.9) | R | OME | N | na | na | + | + | na | + | na | + | + |
Control cochlear implant | |||||||||||||
21 | F (1.6) | L | Sensorineural hearing loss | N | na | na | - | - | - | na | na | - | - |
Positive from number tested (not including healthy control) | 2/17 | 4/9 (44%) | 3/9 (33%) | 15/17 (88%) | 4/10 (40%) | 5/11 (45%) | 7/12 (58%) | 1/1 | 11/17 (64%) | 12/17 (71%) | |||