Representative image of a mucosal biopsy from a child with a history of COME. No middle ear fluid was taken. Child 20. FISH - EUB338 (Yellow), M. catarrhalis (green), S. pneumoniae (pink) and Hoechst 33342 (nuclei stain - blue). Maximum intensity projection (Z = 19.5 μm) showing microcolony formation, mainly M. catarrhalis and S. pneumoniae (arrows) scattered throughout the mucosa. Scale bar = 10 μm.