Figure 4

At age 5 y, during four consecutive whole lung lavage sessions perfluorocarbon (PFC) was instilled into the lungs after the 6th, 7th, or 8th of the 500 ml wash in order to enhance the recovery of surfactant material. The concentration of phosphoplipids (and total protein (not shown)) washed out was not significantly altered compared to lavages done at that age without PFC (Figure. 4 A, B). From age 3 to 4 1/2 years therapy with inhaled and subcutaneous injections of recombinant GM-CSF was done (see also Figure. 2 B). Although the intervals between consecutive therapeutic whole lung lavages were increased with GM-CSF treatment the amount of protein removed was also increased, demonstrating no reduction of protein amount with GM-CSF treatment (Figure. 4 C, D). Note that not all lavages were available for total protein measurements.